Journal: bioRxiv
Article Title: Comparative study of enriched dopaminergic neurons from siblings with Gaucher disease discordant for parkinsonism
doi: 10.1101/2024.02.25.581985
Figure Lengend Snippet: a Confocal image of TH+ DA neurons with and without geneticin treatment. Note the reduction of the nuclei without accompanying TH staining. b Quantification of TH+ DA neurons in ICC (1-way ANOVA, *** p <0.001, n =5 fields of view from 2 independent differentiations). c, d Western blot analysis of TH expression. Data is normalized to cells without geneticin treatment (1-way ANOVA, **p <0.01, n =3 independent differentiations). e Flow analysis of TH+ cells. (t-test, * p <0.05, n =4 independent differentiations).
Article Snippet: On day 10, media was changed to Neurobasal/GlutaMAX/B-27 with 20 ng/ml brain-derived neurotrophic factor (BDNF, Cat.# 450-02, Peprotech), 20 ng/ml glial cell line-derived neurotrophic factor (GDNF, Cat.# 450-10, Peprotech), 200 μM ascorbic acid (Cat#4034, Sigma-Aldrich), 1 ng/ml transforming growth factor type β3 (TGFβ3, Cat.# 243-B3, R&D), 200 μM dibutyryl cAMP (X, Sigma-Aldrich) and 3 μM CHIR99021.
Techniques: Staining, Western Blot, Expressing